plcγ1 tyr 783  (Cell Signaling Technology Inc)

Journal: Investigative Ophthalmology & Visual Science

Article Title: CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression

doi: 10.1167/iovs.64.7.17

Figure Lengend Snippet: Antibodies Used for Immunofluorescence Microscopy

Article Snippet: Phospho-Tyr 783 PLCγ1 (biotinylated) , BS-3343-R , Bioss, Woburn, MA, USA , 1:200 (in vivo).

Techniques: Immunofluorescence, In Vivo, In Vitro

Journal: Investigative Ophthalmology & Visual Science

Article Title: CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression

doi: 10.1167/iovs.64.7.17

Figure Lengend Snippet: Patients with diabetic retinopathy expressed activated PLC γ 1 in Müller cells. Posterior poles from patients with DR, PDR, and non-diabetic controls were incubated with anti-phospho-Tyr783 PLCγ1 (p-PLC) plus anti-vimentin and anti-CD40 antibodies. Arrowheads show some of the areas where phospho-Tyr783 PLCγ1 is co-expressed with vimentin and CD40. Original magnification times 400. Scale bar, 50 µm.

Article Snippet: Phospho-Tyr 783 PLCγ1 (biotinylated) , BS-3343-R , Bioss, Woburn, MA, USA , 1:200 (in vivo).

Techniques: Incubation

Journal: Investigative Ophthalmology & Visual Science

Article Title: CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression

doi: 10.1167/iovs.64.7.17

Figure Lengend Snippet: Model of the changes in expression of CD40, TRAF2, TRAF6, and inflammatory molecules in diabetic retinopathy in humans. Expression of CD40 and its downstream signaling molecules TRAF2 and TRAF6 are increased in retinal Müller cells and endothelial cells from patients with diabetic retinopathy. This is accompanied by increased expression of ICAM-1 in retinal endothelial cells, upregulation of CCL2 in Müller cells, and expression of activated PLCγ1 in Müller cells. TNF-α does not appear to be expressed in Müller or endothelial cells whereas it is detected in cells that likely represent microglia/macrophages. Prior in vitro studies revealed that CD40-TRAF signaling upregulates ICAM-1 in retinal endothelial cells and CCL2 production by Müller cells , as well as activates PLCγ1 in Müller cells, triggering release of extracellular ATP that in turn promotes TNF-α production by myeloid cells . Created with BioRender.

Article Snippet: Phospho-Tyr 783 PLCγ1 (biotinylated) , BS-3343-R , Bioss, Woburn, MA, USA , 1:200 (in vivo).

Techniques: Expressing, In Vitro

Journal: Cell reports

Article Title: Neurokinin-1 Receptor Signaling Is Required for Efficient Ca 2+ Flux in T-Cell-Receptor-Activated T Cells

doi: 10.1016/j.celrep.2020.02.054

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse / human Phospho PLCγ1 (Tyr 783 ) (Clone PLCGTYR783-C4), PE , Invitrogen , Cat # MA5-28030.

Techniques: Functional Assay, Recombinant, Staining, Avidin-Biotin Assay, Blocking Assay, Plasmid Preparation, Inhibition, Fluorescence, SYBR Green Assay, Activation Assay, Bicinchoninic Acid Protein Assay, In Situ, Enzyme-linked Immunosorbent Assay, Activity Assay, Labeling, Software